EPH Receptor B2 anticorps (pTyr331)

N° du produit ABIN361468

Cet anticorps Lapin Polyclonal détecte spécifiquement EPH Receptor B2 dans WB. Il présente une réactivité envers Poulet.

Aperçu rapide pour EPH Receptor B2 anticorps (pTyr331) (ABIN361468)

Antigène: EPH Receptor B2 (EPHB2)

Reactivité: Poulet

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp EPH Receptor B2 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-EPH Receptor B2 anticorps

Épitope: pTyr331

Specificité: Specific for the ~46k EphrinB protein phosphorylated at Tyr331. Immunolabeling is blocked by (-phosphatase treatment.

Réactivité croisée: Souris, Rat (Rattus)

Homologie: bovine, chicken, human, mouse, Xenopus, zebra fish

Purification: Antigen Affinity Purified from Pooled Serum

Immunogène: Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr331 conjugated to KLH

Information d'application

Indications d'application: Recommended Dilution: WB: 1:1000 Quality Control: Western blots performed on each lot.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Stock: -20 °C

Détails sur EPH Receptor B2

Antigène: EPH Receptor B2 (EPHB2)

Autre désignation: EPHB2

Sujet: EphrinB proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development (Flanagan and Vanderhaeghen, 1998, Dufour et al., 2003, Oike et al., 2002). EphrinB molecules expressed at the membrane surface bind to the EphB family receptors on target cells during cell-to cell contact. This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing EphrinB on its surface. This reverse signaling event is thought to be critical for vessel maturation and neuronal development. Importantly, tyrosine phosphorylation of EphrinB is thought to be a critical component of this reverse signaling event (Palmer et al., 2002). Recent work demonstrated that Tyr331 of EphrinB was phosphorylated in HEK293 cells after stimulation by the soluble EphB2 receptor tyrosine kinase (Kalo et al., 2001). Anti-Phospho-Tyr331 EphrinB Western blot of rat testes lysate showing specific immunolabeling of the ~46k EphrinB phosphorylated at Tyr331 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: (-Ptase). The blot is identical to the control except that it was incubated in (-Ptase (1200 units for 30 min) before being exposed to the Anti-Tyr331 EphrinB. The immunolabeling is completely eliminated by treatment with (-Ptase.

Poids moléculaire: '46 kDa

ID gène: 396513

UniProt: P28693

Pathways: Signalisation RTK, Regulation of long-term Neuronal Synaptic Plasticity, S100 Proteins